Polyacrylamide Ability for Protein Immobilization in One- phase Binary- solvent Systems
Kamahldin Haghabeen, Roshanak Agharafeie, Saeed Maghsoudi
Abstract
The protein immobilization ability of cross- linked polyacrylamide (CLP) was studied in one- phase binary- solvent systems. Four miscible solvents in water; 2 protic (methanol, 2 aprotic (THF, acetonitrile), and bovine serum albumin (BSA) as target protein were chosen. According to the results of a study on the sieve bis-acrty amide was selected for the research. Results showed that CLP immobilization efficiency increased as the co-solvent share in the binary-solvents increased. However, it was not necessary to omit water. The immobilization efficiency CLP was satisfying in the binry systems containing about 50% 2-propanol or acetonitrile, or 70% Methanol or THF. The CLP immobilization performance was very high when the organic solvent share was increased above 90% in all the studied binary media. The outcome suggests that the tolerable level of water and the role of co-solvent in the applied system are mainly affect-ed by the amphiphilic nature of CLP. Assumingly, the amphiphilic structure helps CLP to be applied in one- phase binary systems containing co-solvent from various groups of pro-tic or aprotic with different log P.
