en گروه بایومواد Biomaterials Research Paper Research Paper <span style="font-size:12pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Glioblastoma multiforme is an aggressive brain tumor with limited therapeutic options. This study evaluated the multifunctional anticancer effects of curcumin-synthesized silver nanoparticles (curcumin-AgNPs) on the U-87 glioblastoma cell line. Curcumin-AgNPs were biosynthesized using curcumin as a reducing and stabilizing agent and characterized by ultraviolet&ndash;visible spectroscopy (UV&ndash;Vis), dynamic light scattering (DLS), and transmission electron microscopy (TEM). Cytotoxicity was assessed by MTT assay. The mRNA expression of apoptosis- and epithelial&ndash;mesenchymal transition (EMT)-related genes was quantified by real-time PCR. DLS and TEM analyses revealed curcumin-AgNPs with sizes of 56.27&plusmn;4.59 nm and 22&plusmn;3 nm, respectively. Curcumin-AgNPs reduced U-87 MG cell viability in a dose- and time-dependent manner. Analysis of apoptosis-related genes showed an increased <i>BAX/BCL2L1</i> ratio. Additionally, <i>FN1</i> and <i>VIM</i> were downregulated to 0.48- and 0.60-fold, respectively, indicating inhibitory effects on EMT and the metastatic potential of U-87 MG cells. These findings indicated that curcumin-AgNPs exhibit cytotoxic, pro-apoptotic, and EMT-modulating effects in U-87 MG cells, highlighting their potential as a multifunctional nanoplatform for glioblastoma research. Further studies are required to elucidate their underlying mechanisms.</span></span></span> anti-cancer,glioblastoma,silver nanoparticles,curcumin,EMT 56 64 http://ijmse.iust.ac.ir/browse.php?a_code=A-10-5862-2&slc_lang=en&sid=1 Nima Hoseinizadeh nimahzd@gmail.com 1800319475328460021768 1800319475328460021768 No Student research committee, Birjand University of Medical Sciences, Birjand, the Islamic Republic of Iran Farzaneh Kiarad Farzaneh1997@yahoo.com 1800319475328460021769 1800319475328460021769 No Student research committee, Birjand University of Medical Sciences, Birjand, the Islamic Republic of Iran Zahra Kiani kiani.za@gmail.com 1800319475328460021770 1800319475328460021770 No Department of Pharmaceutics and Nanotechnology, School of Pharmacy, Birjand University of Medical Sciences, Birjand, Iran Abolfazl Sadeghi abolfazlsadeghi1998@gmail.com 1800319475328460021771 1800319475328460021771 No Student research committee, Birjand University of Medical Sciences, Birjand, the Islamic Republic of Iran Azadeh Taherpour Taherpoorazadeh@yahoo.com 1800319475328460021772 1800319475328460021772 No Student research committee, Birjand University of Medical Sciences, Birjand, IranCellular and Molecular Research Center, Department of Molecular Medicine, Birjand University of Medical Sciences, Birjand, Iran Mehdi Shakibaie m.shakibaie@bums.ac.ir 1800319475328460021773 1800319475328460021773 Yes Department of Pharmaceutics and Nanotechnology, School of Pharmacy, Birjand University of Medical Sciences