en Research Paper Research Paper Graphene oxide thin layers, graphene oxide:silver nano-composite, graphene oxide:zinc oxide nano-composite and graphene oxide:zinc oxide/graphene oxide:silver bilayer were deposited by spray pyrolysis method. The synthesized thin layers were characterized using X-ray diffraction spectroscopy, field emission scanning electron microscope, <em>energy</em>&nbsp;dispersive x-ray&nbsp;<em>spectroscopy</em> and Raman spectroscopy. The optical properties and the band gap of the thin layers were also studied and calculated using the Tauc equation. Gram-negative bacterium of Escherichia coli was used to study the antibacterial properties of thin layers. The results showed that among the thin layers investigated, GO:ZnO/GO:Ag bilayer had the greatest effect on the inhibition of E. coli growth and was able to control the growth of bacterium after 2 hours. GO:Ag Nano-Composite,Bilayer,Spray Pyrolysis,Thin Layer,GO:ZnO Nano-Composite 170 180 http://ijmse.iust.ac.ir/browse.php?a_code=A-10-2986-1&slc_lang=en&sid=1 S. M. Alduwaib Salmansur1975@gmail.com 1800319475328460012784 1800319475328460012784 Yes Department of Sciences, Collage of Basic Education, Al-Mustansiriyh University, Baghdad Iraq Muhannad M. Abd m20061975@yahoo.com 1800319475328460012785 1800319475328460012785 No Mustansiriyah University, Collage of Basic Education, Department of Science, Baghdad Iraq